Biotin Quantitation Kit

The BroadPharm Biotin Quantitation kit enables a quick estimation of the mole-to-mole ratio of biotin to protein. To quantify biotin label incorporation, a solution containing the biotinylated protein is added to a mixture of HABA and avidin. Because of its higher affinity for avidin, biotin displaces HABA from its interaction with avidin and the absorption at 500 nm decreases proportionately. By this method, an unknown amount of biotin present in a solution can be evaluated in a single cuvette by measuring the absorbance of the HABA-avidin solution before and after addition of the biotin-containing sample. The change in absorbance relates to the amount of biotin in the sample.

For your biotinylating needs refer to BroadPharm kits Sulfo-NHS-Biotin (BP-50056) and NHS-PEG4-Biotin (BP-50054)