Name: BP Fluor 647 NHS Antibody Labeling Kit (1 x 100 ug)
Brand: BroadPharm
SKU: BP-50029
Price: 510.00 USD
Availability: InStock

BP Fluor 647 NHS Antibody Labeling Kit

Catalog #: bp-50029

Overview

BP Fluor 647 is a far-red bright and photostable fluorophore absorbed at 650 nm andemitted at 671 nm. BP Fluor 647 is water soluble and pH-insensitive from pH 4 to pH 10. The sulfonate groups make it high water soluble and less aggregation in the aqueous solution.
BP Fluor 647 is spectrally similar to Cy5, Cyanine 650. These three dyes, BP Fluor 647, Cyanine 650, and Cy5, are bright green-fluorescent dye optimal for use with the 633nm, 640nm 650 nm argon laser.
BP Fluor 647 NHS antibody labeling kit is used for protein and antibody labeling, or nucleic acid applications with high labeling density for applications such as immunolabeling, fluorescence microscopy, and flow cytometry.
Components
All three dyes can be conjugated to primary antibody, secondary antibody, or other biomolecules for the application below.
- Flow cytometric analysis of antibody surface-stained cells (FC)
- Flow cytometric analysis of intracellularly-stained cells ( ICFC)
- Analysis of antibody-labeled tissue preparations, typically frozen or paraffin-embedded. (IHC-F)
- IHC-P
- Immunocytochemistry is a technique used to assess the presence of a specific protein or antigen in cells (cultured cells, cell suspensions) by use of specific antibodies, thereby allowing visualization and examination under a microscope. The readout can be colorimetric using chromogen substrate or fluorescent using fluorophore-conjugated antibodies. (ICC)
- Immunofluorescence Microscopy, Microscopic examination of fluorescently-labeled cells, either grown on or spun onto slides, or tissue preparations (IF)
- Immunodetection applied to formalin-fixed tissue sections of at least 300 microns in thickness, which are optically cleared by tissue clearing reagent (3-DIHC)
- IHC
- WB
Application

bp fluor647_cy5_cyanie650*delimiter*general_protocol_dye_mal_labeling_kit*delimiter*general_protocol_dye_nhs_labeling_kit
Protocol

BP Fluor647 Cy5 Cyanie650 General protocol dye MAL labeling kit General protocol dye NHS labeling kit

BP Fluor 647 NHS Antibody Labeling Kit

BP Fluor 647 NHS Antibody Labeling Kit

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Overview

Components

Application

Protocol

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